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KMID : 1234420120400040380
Korean Journal of Microbiololgy and Biotechnology
2012 Volume.40 No. 4 p.380 ~ p.388
Antibacterial Properties Associated with Microorganisms Isolated from Arctic Lichens
Kim Mi-Kyeong

Park Hyun
Oh Tae-Jin
Abstract
A total of 5 different polar microorganisms were isolated from Arctic lichens and their bioactive compounds were extracted from cell culture using different solvents including acetone, water, chloroform, diethylether, ethanol, ethyl acetate, methanol, and petroleum ether. The antibacterial properties of the extracts were evaluated by disk diffusion tests and minimal inhibitory concentration tests against 6 bacterial pathogens; Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus, Enterobacter cloacae, Pseudomonas aeruginosa and Escherichia coli. Among the extraction samples, ethyl acetate extracts of Burkholderia sordidicola S5-BT (KOPRI 26644) showed the highest activity (inhibition zone, 7-10 mm; MIC value, 57.5-1000 ug/ml) against targeted bacteria. Among the various solvents used for extraction, chloroform extract exhibited the weakest, but still obvious, activity.
KEYWORD
Antibacterial activity, paper disk diffusion test, lichen, minimal inhibitory concentration test, Polar microorganisms
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